Figure 5

Blocks to entry and reverse transcription are not dissociable from one another. MDM from the same donor were infected with equal amounts of p24 VSV-G glycoprotein pseudotyped YU-2, CH058 and CH077 viruses. (A) MDM were fixed and stained for intracellular p24 protein 48 h post-infection. (B,C) MDM were harvested and total DNA was isolated at 0 h, 6 h and 18 h post-infection. Viral DNA products were detected using quantitative PCR. (B) total DNA; (C) strong stop. This is a representative example of three independent experiments on different donors.