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Fig. 2 | Retrovirology

Fig. 2

From: Six-helix bundle completion in the distal C-terminal heptad repeat region of gp41 is required for efficient human immunodeficiency virus type 1 infection

Fig. 2

The protein profiles of Env mutants. The expression and processing of Env were analyzed by immunoblotting. Representative results of immunoblotting analysis of Env with NHR and loop mutants (a) and CHR mutants (c) expressed in the transfected 293FT cells probed with goat anti-gp120 or with Chessie 8 anti-gp41 antibodies are shown. The gp160 and gp41 bands were then quantified (b and d) by measuring the intensity of bands on the anti-gp41 immunoblot. The expression levels were normalized to that of the wild-type protein. Statistical analysis was performed using one way ANOVA. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05; ns indicates no statistically significant difference when compared with the WT protein

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Retrovirology

ISSN: 1742-4690

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