Fig. 1

Characterisation of FISH-Flow technique in J-Lat cells. J-Lat cells were either treated with DMSO or with PMA to reactivate the provirus. Jurkat cells were used as an uninfected negative control. a Dot plots representing cells gates for size by forward and side scatter, for singlets by forward scatter height versus area and finally for GFP expression and vRNA staining. b The % of GFP+ and the % of vRNA-expressing cells were quantified. Error bars represent the standard deviation from three independent experiments. c Representative images of cells in each of the above conditions imaged by confocal microscopy. In example images from sorted populations, DAPI is in blue, vRNA in red, and cells making viral protein produce GFP in green. Scale bars represent 10 μm