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Fig. 3 | Retrovirology

Fig. 3

From: Engineering multi-specific antibodies against HIV-1

Fig. 3

Secretion of CrossMAb byproducts that could hinder cell line development and clone selection. Detection of the indicated antibody or antibody byproduct in supernatant after transient transfection of ORFs encoding for the antibody chains indicated in the schematics. Protein detection in supernatant was determined by Protein A binding ELISA. Dashed lines indicate the assay limit of detection. Error bars = SD. a HH dimer byproducts and b HHL impurity byproducts were readily detected in supernatants

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