Fig. 4

Impact of knockdown of PSF on HIV replication as measured by luciferase reporter gene assay. a, b are siRNA knockdown study at 100 and 150 nM respectively to achieve maximum knockdown. c Luciferase activity observed at 24 and 48 h at 1 MOI. TZM-bl cells were transfected with a pool of PSF siRNA (siPSF) at 150 nM and control siRNA (siScrambled) and viral replication was monitored. Data depicted here shows average values ± SD of 3 independent experiment (**p < 0.02). Knockdown was measured at every step