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Fig. 6 | Retrovirology

Fig. 6

From: Interaction of HIV-1 integrase with polypyrimidine tract binding protein and associated splicing factor (PSF) and its impact on HIV-1 replication

Fig. 6

Evaluating the impact of PSF on different steps of virus life cycle. a Quantitative PCR to detect the formation of early RT product at different time point. Error bar depicts SD between three independent experiment (p > 0.05). b To examine the virus infectivity produced in PSF knockdown cells—Supernatant collected from PSF knockdown (siPSF) and scrambled (siScambled) cells were used to infect the TZM-bl cell line. The infective virus particle were calculated. No significant difference in virus infectivity was observed. Data depicted here shows average values ± SD of 3 independent experiment (p > 0.05). c Western blot to detect the PSF incorporation in the cells and virus of control cells by using 150 ng of protein and PSF was detected by anti-PSF antibody

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Retrovirology

ISSN: 1742-4690

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