Fig. 1

Illustrated library preparation and bioinformatics pipeline. A DNA from outbred (n = 7), hybrid colony (n = 6), and SPF colony (n = 7) domestic cats was sheared and ligated to synthesized linkers to selectively amplify enFeLV-LTR integration sites. Due to library preparation methods, integration sites displayed a Poisson distribution. B Bioinfomatics pipeline used to generate mapped LTR integration identified unique and common integration sites shared between all cats included in this study