Fig. 2

HIV-1 Gag-fusion viruses activate a restrictive type I IFN response in primary macrophages. A-C: ISG qRT-PCR from primary MDM transduced for 24 h with 0.5 U RT/ml LAI or Gag-LUC viruses, or stimulated with 1 ng/ml IFNβ as a control, in the presence of DMSO vehicle or 2 µM ruxolitinib. D: CXCL-10 protein in supernatants from A-C (ELISA). E: Infection data from A-D measured by flow cytometry at 48 h. Data are mean ± SD from biological triplicates of a single experiment, representative of at least 3 repeats. Statistical analyses were performed using Student’s t-test, with Welch’s correction where appropriate, comparing pairs of samples -/+ ruxolitinib as indicated. *P < 0.05, **P < 0.01, ***P < 0.001