Fig. 3
From: HIV-1 with gag processing defects activates cGAS sensing
ISG induction by HIV-1 Gag-fusion virus is RT-dependent. A: IFIT-1 reporter activity from monocytic THP-1-IFIT-1 cells transduced for 24 h with Gag-LUC, RT-defective Gag-LUC (Gag-LUC RT D185E) or integrase-defective Gag-LUC (Gag-LUC INT D116N) at 1.25 × 109, 2.5 × 109 and 5 × 109 genomes/ml. B, C: ISG qRT-PCR from PMA-treated THP-1 shSAMHD1 cells transduced for 24 h with Gag-LUC, Gag-LUC RT D185E or Gag-LUC INT D116N at 1.25 × 109, 2.5 × 109 and 5 × 109 genomes/ml. D: CXCL-10 protein in supernatants from B, C (ELISA). E: IRF reporter activity from THP-1 Dual cells transduced for 24 h with 8.91 LAI or Gag-Luc (1.5 U RT/ml) in the presence of DMSO vehicle, 5 µM neviripine or 10 µM raltegravir. F, G: ISG qRT-PCR from THP-1 Dual cells transduced for 24 h with 8.91 LAI or Gag-Luc (1.5 U RT/ml) in the presence of DMSO vehicle, 5 µM neviripine or 10 µM raltegravir. Data are mean ± SD from biological triplicates of a single experiment, representative of at least 3 repeats. Statistical analyses were performed using Student’s t-test, with Welch’s correction where appropriate, comparing mutant viruses with WT Gag-LUC at the same dose (A-D) or to the DMSO control as indicated (E-G). *P < 0.05, **P < 0.01, n.s. non-significant