Fig. 3

HIV infection induces neuronal loss in the frontal cortex and spinal cord. Quantification of NeuN⁺ neurons with representative images of NeuN⁺ neurons (green, Hoechst/cell nuclei in blue) for all five BLT humanized mouse groups in the frontal cortex (A) and spinal cord (B). Statistical significance was assessed by one-way ANOVAs followed by Bonferroni’s post hoc tests when appropriate; *p < 0.05, **p < 0.01, ***p < 0.001. Sample derived from 5–8 sections per mouse with n = 4 mice per group. NeuN: neuronal nuclear protein. Scale bars = 20 μm