Fig. 3

RSV Gag colocalized and co-immunoprecipitated with Med26. A Transfected RSV Gag-GFP (red) and FLAG-Med26 (green) colocalize (white) within QT6 cells. Image representative of average colocalization, as quantified in panel (B). Nuclei (blue) are outlined by a dotted white line, and regions boxed in the main images are enlarged below. White arrows are included to guide the eye. Scale bars = 2 µm. B Manders’ Overlap Coefficient values for image set represented by (A). Individual values are shown in addition mean ± SEM, n ≥ 17; ****, p < 0.0001 by unpaired two-tailed t-test. C 500 µg of RC.V8-infected QT6 nuclear lysates were incubated with an α-RSV Gag antibody (mouse α-RSV CA.A11, gift from Neil Christensen, Penn State College of Medicine), followed by antibody capture on Pierce™ Protein G Magnetic Beads. After extensive washing, proteins were eluted from beads by boiling in 1X SDS-PAGE sample buffer and run on a 10% SDS-PAGE gel, transferred to PVDF, and Western blotted first for Med26 (top) followed by RSV Gag (bottom). The position of molecular weight markers, in kilodaltons, are indicated on the left. FT, flow through; Beads, lysate only; Eluate, lysate plus antibody. Images representative of three independent experiments