Fig. 4
From: High level of genomic divergence in orf-I p12 and hbz genes of HTLV-1 subtype-C in Central Australia
Key mutations in HTLV-1c orf-I p12 and hbz may facilitate alternate splicing and divergent protein structures. A Three-dimensional (3D) modelling of HTLV-1a (red) and HTLV-1c (yellow) orf-I product p12 based on the translated amino acid sequences of subtype-A (AB513134.1, AB979451.1, AF033817.1, AF042071.1, AF139170.1, HQ606137.1, HQ606138.1, KC807984.1, L03562.2, L36905.1, U19949.1) and subtype-C (P9-31, Aus-CF, Aus-DF, Aus-NR, Aus-GM, MEL5) consensus genomes. Star (*) represents an absence of p12 initiation codon, present in 100% of the sequences, due to a substitution AUG (Methionine) to ACG (Threonine). Black lines indicate regions of structural alignment. B Alternate mRNA splicing with rex exon 1, containing an intact start codon (AUG), and an alternate second acceptor site upstream of the equivalent HTLV-1a orf-I p12 site, is predicted to facilitate the expression of an HTLV-1c p16 product. C Comparative 3D modelling of HTLV-1a p12 (red) and putative HTLV-1c p16 product (blue). Black lines indicate regions of structural alignment.). D Alignment of HTLV-1a and HTLV-1c HBZ 3D protein structures, where regions of structural homology are indicated by black lines