Fig. 2

Impact of PBMC preparation on the detection of positive HERV loci. A scRNA-seq dataset of paired PBMC samples from the same donor that were prepared from fresh (not frozen) and those samples from the same donor that were frozen prior to use [26]. We sorted sequences for CD14 + monocytes and analyzed output file to conduct the WHA. A Heatmap of positive HERV loci detected from PBMC. Individual specific transcribed HERV loci the repeat samples (rep1A, rep1B, and rep2A rep 2B) are presented. The percent similarity for the paired fresh samples: 82.5–84.2% and frozen samples: 92.9–100%. B Phylogenetic tree of the PBMC prepared fresh after isolation versus frozen. phylogenetic tree shows the individual similarity of the repeat samples and differences between fresh and frozen from the same individual. C Clustering of positive HERV loci from fresh or frozen sample sets. A PCA of illustrates the clustering between the fresh and frozen samples and highlighting the individual specificity of the HERV loci identification