Fig. 5
From: Expression of HIV from a 1-LTR circular DNA in the absence of integration
Production of infectious virus from Jurkat cells transfected with 1-LTRHIV. (A) Syncytia were observed 3 days after transfection. At 4 days, the culture supernatant contained 716 ng/ml of p24. (B) Expression of Tat activity in TZM cells incubated with the supernatant of Jurkat cells transfected with 1-LTRHIV. TZM cells were infected with 1 ml of culture supernatant containing 316 ng of p24 and processed daily for quantification of luciferase activity. Three days after infection 3.5 × 107 RLU (relative luminescence units) were measured versus 1.5 × 104 in the untreated control. (C-D) Infection of Jurkat cells with the supernatant of the transfected Jurkat cells. As is the case with transfected cells, a large number of syncytia was observed as shown. Four days after infection the level of p24 in the culture medium was 141 ng/ml