Fig. 7

Intracellular and soluble p24 in HeLa-tat cells transfected with 1-LTR_HIV. (A) Flow cytometry analysis of HeLa-tat cells transfected with 1-LTR_HIV. The absolute number of cells stained intracellularly for p24 was determined by Trucount analysis, as described in the Materials and methods. A representative plot showing untransfected cells and p24⁺ cells detected at different timepoints is shown (upper panels). Since Trucount beads fluoresce at multiple wavelengths, data was plotted using two fluorescence detectors (FL-1 and FL-2) to facilitate gating of both Trucount beads and p24⁺ cells. (B) Growth rate of the transfected and intracellularly stained HeLa-tat cells. The growth rate of the transfected HeLa-tat cells (bulk culture) and that of the subpopulation of p24⁺ cells contained in the bulk culture are represented. As shown, the subpopulation of p24⁺ cells grows at a lower rate than that of the bulk culture. (C) Trucount values and mean fluorescence intensity of p24 stained cells. Data are plotted as a function of time. The data reported in (B) and (C) are the average of two experiments; error bars represent one standard deviation. (D, E) Intracellular and soluble p24 in HeLa-tat cells transfected with 1-LTR_HIV. (D) The level of p24 in the culture supernatant of the transfected cells and the number of p24⁺ cells, measured at daily intervals, are shown. (E) The ratios of soluble p24and the number of p24⁺ cells are plotted as a function of time