Fig. 7
From: Dysregulation of the tumor suppressor Menin and its target Bach2 in HTLV-1 infection
HBZ and Menin exhibited different subcellular localization between MT-2 and SLB-1, which shows opposite effects on cell proliferation by Menin-MLL interaction inhibitor. The subcellular localization of Menin and Bach2 in SLB-1 and MT-2 cells was determined and compared with that of the HTLV-1 regulatory factors Tax and HBZ by indirect immunofluorescence staining. Tax was localized in the cytoplasm of both MT-2 and SLB-1 cells, whereas HBZ showed different localization patterns in both cell lines. (A) HBZ was predominantly localized in the nucleus of MT-2 cells. Menin was mainly localized in the nuclei of MT-2 cells. Bach2 is localized in the cytoplasm of MT-2 cells. (B) HBZ is predominantly localized in the cytoplasm of SLB-1 cells. Menin was predominantly localized in the cytoplasm of SLB-1 cells. Bach2 is localized in the cytoplasm of SLB-1 cells. HBZ and Bach2 were partially co-localized in the cytoplasm of SLB-1 cells (white arrow)