Fig. 1

(A) HT1080 cells were treated with Mock or PFV (MOI = 0.5) for 48 h. Western blotting was performed to assess changes in the levels of p62 and LC3 II/I proteins. (B-C) Statistical analysis of the relative gray intensity of p62 and LC3 II/I was conducted, using Student’s t-test (n = 3, ***p < 0.001, *p < 0.05). (D) Following PFV infection of HT1080 cells at various time points (0 h, 24 h, 48 h), cells were incubated with 5 µM MitoSOX, protected from light, for 10 min. Fluorescence intensity changes were quantitatively analyzed using flow cytometry. (E) Statistical analysis of MitoSOX ROS fluorescence intensity is shown (n = 4, ***p < 0.001, ns p > 0.05). (F) After PFV infection of HT1080 cells for 0 h, 24 h, and 48 h, mitochondrial isolation was performed, and Western blotting was used to evaluate changes in p62 and LC3 II/I protein levels in both cytoplasmic and mitochondrial fractions. (G-H) The ratios of p62 and LC3 II/I protein content in the cytoplasm versus their respective content in mitochondria were statistically analyzed (Student’s t-test, n = 3, ***p < 0.001, **p < 0.01, *p < 0.05)