Fig. 3-2

(I) Statistical analysis of the fluorescence intensity of Tom20 (Student’s t-test, n = 30, *p < 0.05) (J) After constructing a shAtg5 plasmid to knock down Atg5, HEK293T cells were cotransfected with the shAtg5 and pCDNA6.0-His-Gag plasmid for 48 h. The protein expression levels of Tom20 and Cox4 in the cell lysate were subsequently detected. (K-L) Statistical analysis of Tom20 and Cox4 protein expression levels was performed (One-way ANOVA, n = 3, *p < 0.05, **p < 0.01, ns p > 0.05)